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Fig. 1 Ntl and Tbx16 proteins are co-expressed in DFCs. (A–I) Confocal microscopy of double fluorescent immunostaining in wild-type embryos using anti-Tbx16 (green) and anti-Ntl (red) antibodies. Ntl and Tbx16 co-localized in the nuclei of margin cells and dorsal forerunner cells (DFCs; arrows in panels A–F) at 60% epiboly (A–C) and 90% epiboly (D–F) stages. At 4 SS, Tbx16 expression (G) was detected in tailbud cells surrounding Kupffer's vesicle (KV), but not in KV cells marked by Ntl expression (arrow head in panels H, I).
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Reprinted from Developmental Biology, 310(2), Amack, J.D., Wang, X., and Yost, H.J., Two T-box genes play independent and cooperative roles to regulate morphogenesis of ciliated Kupffer's vesicle in zebrafish, 196-210, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.