IMAGE

Fig. 4

ID
ZDB-IMAGE-070822-61
Genes
Source
Figures for Pei et al., 2007
Image
Figure Caption

Fig. 4 Temperature affects Nodal activity. (A–L) Temperature increase causes reductions in Nodal target gene expression. WT (A–F) and MZsqthi975/hi975 (G–L) embryos from single respective clutches were incubated either at 28 °C from 0 to 6 h (A, C, E, G, I, K) or 28 °C from 0–1.75 h and 34 °C from 1.75 to 5.3 h (B, D, F, H, J, L), then collected and fixed when shield formation was apparent and probed for cyc (A,B,G,H) ([Rebagliati et al., 1998a] and [Sampath et al., 1998]), goosecoid (C,D,I,J) (Stachel et al., 1993) or bhikhari (E,F,K,L) (Vogel and Gerster, 1999) expression by whole-mount in situ hybridization. (M–N) Temperature shifts cause reductions in Nodal activity. WT and MZsqthi975/hi975 embryos from single respective clutches were injected with 25 pg of luciferase reporter plasmids, incubated at 34 °C or 15 °C for 1 h starting at the 256-cell stage (2.5 hpf), or kept at 28 °C, then collected at shield stage and measured for luciferase activity. Significant reductions in activity were seen in MZsqthi975/hi975 embryos subjected to heat or cold, as well as WT embryos subjected to cold.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 308(2), Pei, W., Williams, P.H., Clark, M.D., Stemple, D.L., and Feldman, B., Environmental and genetic modifiers of squint penetrance during zebrafish embryogenesis, 368-378, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.