ZFIN ID: ZDB-GENE-980526-485
Gene Name: POU domain, class 5, transcription factor 3
Gene Symbol: pou5f3    Nomenclature History

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Previous Names: oct4, pou2 (1), pou5f1 (1), cb197 (1), chunp6868, etID49452.21 (1), gp-9, gp9, spg, spiel ohne grenzen, spiel-ohne-grenzen (1), wu:fd18d06

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(Including Attributions)
Gene Type: protein_coding_gene
Location: Chr: 21 Mapping Details/Browsers
Description: Exhibits several functions, including DNA-binding transcription factor activity; chromatin binding activity; and protein heterodimerization activity. Involved in several processes, including brain development; embryonic morphogenesis; and regulation of nucleobase-containing compound metabolic process. Predicted to localize to the nucleus. Human ortholog(s) of this gene implicated in embryonal carcinoma and seminoma. Is expressed in several structures, including blastoderm; blastodisc; ectoderm; gonad; and hindbrain neural keel. Orthologous to several human genes including POU5F1 (POU class 5 homeobox 1) and POU5F1B (POU class 5 homeobox 1B).
Genome Resources: Alliance (1),  Gene:30333 (1),  Ensembl(GRCz11):ENSDARG00000044774 (1)
Note: The gene name was updated on May 20, 2013 to reflect the evolutionary relationship of this gene with the mammalian POU domain, class 5 genes. The data suggests that there is not a true ortholog in human and mouse for this zebrafish gene. The synteny, amino acid alignment and phylogenetic trees suggest that a duplication event occurred in the last common ancestor of extant cartilaginous fishes and bony fishes resulting in 2 POU5 genes. Subsequently, the bony fishes, marsupials and birds maintained pou5f3, but lost pou5f1 which was maintained in eutherians and marsupials. This gene is an apparent ortholog (AA, CL) with the opposum, xenopus and chicken Pou5f3 genes.
MUTATIONS AND SEQUENCE TARGETING REAGENTS
Allele Type Localization Consequence Mutagen Suppliers
e68 Unknown Unknown Unknown ENU
e713 Point Mutation Unknown Missense ENU
hi349Tg Transgenic Insertion Exon 3 Unknown DNA
  • Zebrafish International Resource Center (ZIRC) (order this)
  • hi1757Tg Transgenic Insertion Unknown Unknown DNA
  • Zebrafish International Resource Center (ZIRC) (order this)
  • hi1940Tg Transgenic Insertion Exon 1 Unknown DNA
  • Zebrafish International Resource Center (ZIRC) (order this)
  • ihb137 Small Deletion Unknown Unknown CRISPR
    ihb138 Small Deletion Unknown Unknown CRISPR
    la013973Tg Transgenic Insertion Unknown Unknown DNA
  • Zebrafish International Resource Center (ZIRC) (order this)
  • m216 Point Mutation Unknown Missense ENU
  • Zebrafish International Resource Center (ZIRC) (order this)
  • m308 Point Mutation Acceptor Splice Site of Exon 5 Frameshift ENU
    m793 Point Mutation Acceptor Splice Site of Exon 2 Frameshift, Premature Stop ENU
  • Zebrafish International Resource Center (ZIRC) (order this)
  • pou5f3_unspecified Unspecified Unknown Unknown
    sa1294 Point Mutation Unknown Premature Stop ENU
    sa1329 Point Mutation Unknown Premature Stop ENU
  • Zebrafish International Resource Center (ZIRC) (order this)
  • wi270Tg Transgenic Insertion Unknown Unknown DNA
    Sequence Targeting Reagents
    Targeting Reagent Created Alleles Publications
    CRISPR1-pou5f3 1
    MO1-pou5f3 N/A 8
    MO2-pou5f3 N/A 2
    PHENOTYPE
    Data: 54 figures from 18 publications
    Observed in:
    DISEASE ASSOCIATED WITH pou5f3 HUMAN ORTHOLOG No data available
    DISEASE ASSOCIATED WITH pou5f3 VIA EXPERIMENTAL MODELS No data available
    GENE ONTOLOGY
    Ontology GO Term
    Biological Process positive regulation of transcription, DNA-templated (more)
    Cellular Component nucleus (more)
    Molecular Function chromatin binding (more)
    GO Terms (all 29)
    PROTEIN FAMILIES, DOMAINS AND SITES
    Type InterPro ID Name
    Domain IPR000327 POU-specific domain
    Domain IPR001356 Homeobox domain
    Homologous_superfamily IPR009057 Homeobox-like domain superfamily
    Homologous_superfamily IPR010982 Lambda repressor-like, DNA-binding domain superfamily
    Domain IPR013847 POU domain
    Family IPR015585 POU domain-containing protein, class 5
    Conserved_site IPR017970 Homeobox, conserved site
    TRANSCRIPTS
    Type Name Length (nt) Analysis
    mRNA pou5f3-201 (1)    Ensembl 2476
    Browsers: UCSCNCBIEnsemblZFIN
    INTERACTIONS AND PATHWAYS
    ANTIBODIES
    Name Type Isotype Host Organism Assay Source Publications
    Ab3-pou5f3 polyclonal Rabbit WB 1
    Ab2-pou5f3 polyclonal Rabbit IHC, WB 1
    PLASMIDS No data available
    CONSTRUCTS WITH SEQUENCES FROM pou5f3
    Construct Regulatory Regions Coding Sequences Species Tg Lines Publications
    Tg(-6.5pou5f1:EGFP) pou5f3 EGFP Danio rerio 1 4
    Tg(hsp70l:Dme.En-pou5f1) hsp70l pou5f3 Danio rerioDrosophila melanogaster 1 3
    MARKER RELATIONSHIPS
    pou5f3 Contained in: [BAC] CH73-205E12 (1)
    pou5f3 Contains: [SNP] rs3729086 (1)
    pou5f3 Encodes: [EST] cb197 (1) (order this), fd18d06
    [STS] chunp6868
    [cDNA] MGC:55202 (1)
    SEQUENCE INFORMATION
    Type Accession # Length (bp/aa) Analysis
    RNA RefSeq:NM_131112 (1) 2425 bp
    Genomic GenBank:FP089537 (1) 98611 bp
    Select Tool
    Polypeptide UniProtKB:A0A0R4I9C7 (1) 472 aa
    Sequence Information (all 26)
    ORTHOLOGY for pou5f3 ( Chr: 21 )
    CITATIONS (162)