Fig. 6

Cytokine IL-1β induction of Müller glia proliferation does not require IL-10. (A–D) Confocal images of undamaged albino;Tg(gfap:EGFP)nt11 zebrafish retinas that were intravitreally injected with IL-1β alone (A), or electroporated with il-10 morpholino and intravitreally injected with either MO only (B), PBS (C), or IL-1β protein (D). Sections were collected at 3 days post-injection (dpi) and immunostained for GFP (Müller glia, green) and PCNA (proliferating cells, magenta), with DAPI counterstain (nuclei, blue). (E) Quantifications of the numbers of PCNA+ INL cells under different conditions. (F) Quantifications of the numbers of PCNA+ ONL cells under different conditions. Quantifications were normalized to 300 μm along the central-dorsal retina. Statistical analyses were performed using one-way ANOVA followed by Tukey’s post hoc test. Mean ± SEM and n ≥ 9, **, p < 0.01; ****, p < 0.0001; ns, not significant. ONL, outer nuclear layer, INL, inner nuclear layer, GCL, ganglion cell layer. Scale bar in A is 20 μm and is the same for (B–D).