Fig. 1

Treatment with PLX3397 and Dexamethasone reduces the number of microglia and proliferating Müller glia in light-damaged retinas. (A–H) Confocal images of albino;Tg(gfap:EGFP)nt11 retinas that were light-damaged and collected at 0, 24, 36, and 72 h of light treatment (LT). Eyes were intravitreally injected with either DMSO (A–D) or PLX3397 and Dexamethasone (PLX + Dex, E-H) at 0 h LT. Sections were immunostained to detect GFP (Müller glia, green), PCNA (proliferating cells, magenta), and Lcp1 (microglia, white), with DAPI counterstain (nuclei, blue). (I) Quantifications of the numbers of Lcp1+ ONL cells under the different conditions described above. (J,K) The numbers of PCNA+ cells were quantified in the INL (J) and ONL (K). Quantifications were normalized to 300 μm along the length of the central-dorsal retina. Statistical analyses were performed using two-way ANOVA with Bonferroni’s post hoc test. Graphs represent the Mean ± SEM and n ≥ 9. ***, p < 0.001, ****, p < 0.0001. ONL, outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar in A is 20 µm and is the same for (B–H).