Figure 6

EDA promotes AHR and NRF2 pathway activation in zebrafish larvae. (a) cyp1a, ahrra and ahrrb transcript expression in zebrafish larvae at 56 hpf treated with vehicle (DMSO) or 10 or 30 µM EDA for 24 and 48 h. Asterisks above bars indicate statistically significant changes compared to DMSO-treated (control) samples. (b) Representative Western blot for the eGFP reporter protein on fish trunk whole lysates from control DMSO and EDA-treated Tg(8x AORE:EGFP)^ia201 larvae at 56 hpf. Fish were treated for 48 consecutive hours. For both gene expression and Western blot analysis, data are expressed as the mean ±SEM of 4 biological replicates (10 larvae per replicate). (c,d) Representative Western blot for Nrf2 and Gclc proteins on fish trunk whole lysates from control DMSO and EDA-treated larvae at 56 hpf. Data are expressed as the mean ± SEM of 6 biological replicates (10 larvae per replicate). * p < 0.05, ** p < 0.01 and *** p < 0.001 with unpaired Student’s t-test. The image was edited using BioRender.com (https://www.biorender.com/ accessed on 3 April 2024).