FIGURE

Fig. 2

ID

ZDB-FIG-240130-13

Publication

Yang et al., 2023 - Optimized protocol for live imaging of overnutrition-elicited interactions between immune cells and β cells in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Prepare and use the nutrient-rich medium (A) Harvest egg yolk. (B) Add equal amount of 0.3x Danieau’s solution to an egg yolk-containing conical tube. (C) Generate 50% stock by vigorous vortexing. (D) Prepare working medium by vigorous mixing of 5 mL Stock and 45 mL 0.3x Danieau’s solution. (E) Add 25 mL of the 5% egg yolk medium and < 20 larvae to a clean 50 mL beaker. (F) Transfer the larvae into the Sterile Cell Strainer in a Petri dish. (G) Quickly transfer the larvae into a 50 mL clean beaker with 25 mL of the 5% egg yolk medium. (H) Put the beaker into a 28°C incubator. As sediments accumulate, stir the medium every 2 h (I) Transfer the larvae clear of egg yolk into a Petri dish with 0.3x Danieau’s solution.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ STAR Protoc