FIGURE

Fig. 4

ID

ZDB-FIG-231109-12

Publication

Yu et al., 2023 - Cell competition for neuron-derived trophic factor controls the turnover and lifespan of microglia

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Alteration of neuron-derived Il34 level changes the proportion and lifespan of RBI microglia. (A) Schematic diagram shows the experimental setup for RBI microglia tracing in WT and Tg(Xla.Tubb:il34) fish. (B) Representative images of the midbrain cross section of 4-OHT–treated Tg(mfap4:creER;mpeg1:LRLG) and Tg(Xla.Tubb:il34;mfap4:creER;mpeg1:LRLG) fish at 6 mpf. GFP+ and GFP−DsRed+ cells represent RBI and AGM microglia, respectively. (C and D) Quantification of the number (C) and proportion (D) of RBI (GFP+) and AGM (GFP−DsRed+) microglia in (B) (n = 3 for each genotype). (E) Schematic diagram shows the experimental setup for RBI microglia tracing in siblings and il34−/− mutants. (F) Representative images of the midbrain cross section of 4-OHT–treated Tg(mfap4:CreER;mpeg1:LRLG) siblings and il34−/−;Tg(mfap4:CreER;mpeg1:LRLG) mutant fish at 2 mpf. GFP+ and GFP−DsRed+ cells represent RBI and AGM microglia, respectively. (G and H) Quantification of the number (G) and proportion (H) of RBI (GFP+) and AGM (GFP−DsRed+) microglia in (F) (sibling n = 6, il34−/− n = 5). n.s., P > 0.05; *P < 0.05; ***P < 0.001; ****P < 0.0001 (Student’s t test). Data were presented as mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci Adv