FIGURE

Fig. 8.

ID

ZDB-FIG-231002-111

Publication

Leid et al., 2023 - Deletion of taf1 and taf5 in zebrafish capitulate cardiac and craniofacial abnormalities associated with TAFopathies through perturbations in metabolism

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Fig. 8.

Defective oxidative metabolism in cora^stl325 and taf1 knockout embryos. (A) RTPCR showing the reduced expression of genes involved in the TCA cycle (Asdph, Suclg2) and the electron transport chain (Aifdm5, Cox4il) in cora^stl325 and taf1^stl456 embryos at 96 hpf. Each data point represents a pool of 20 embryos. (B) Reduced basal oxygen consumption rates (OCR) in cora^stl325 (n=12) and taf1^stl456 (n=12) embryos compared to controls at 96 hpf (left). Preserved basal OCR in ttn^{stl853/stl853} (n=20) compared to controls (n=18) embryos at 96 hpf (right). Data were obtained from a Seahorse XF24 Bioanalyzer. (C) Targeted metabolite assays measuring NAD/NADH (left) and ADP/ATP (right) in control, cora^stl325 taf1^stl456, and ttn^{stl853/stl853} embryos at 96 hpf. n=4 pools of 100 embryos per genotype. (D) Oxygen consumption rates of isolated mitochondria for fatty acid metabolism. n=4 pools of 100 embryos per genotype

Expression Data

Genes:	aifm5 aspdh cox4i1l suclg2
Fish:	WT taf1^{stl456/stl456} taf5^{stl325/stl325}
Anatomical Term:	whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	taf1^{stl456/stl456} taf5^{stl325/stl325}
Observed In:	aerobic respiration whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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