FIGURE
Fig. 3
- ID
- ZDB-FIG-230906-15
- Publication
- Arias-Alpizar et al., 2022 - Phase-separated Liposomes Hijack Endogenous Lipoprotein Transport and Metabolism Pathways to Target Subsets of Endothelial Cells in vivo
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Fig. 3
Cryo-TEM of PAP3 liposomes characterized by phase separation correlates with the bECs targeting in zebrafish larvae (≈78 hpf). a,b) CryoTEM of PAP3 (50–50 mol% DOaG-DSPC) liposomes and c) 3D model of a representative PAP3 liposome reconstructed based on the electron density derived from cryo-electron tomography, demonstrating the whole body and different plane sections of the particle. CryoTEM and biodistribution (in a Tg(kdrl:GFP) zebrafish embryo at 1.5 hpi) of liposomes containing DSPC and d,e) DOaG 10 mol%, f,g) 20 mol%, and h,i) 30 mol%. Liposomes (5 mM, 0.2% mol DOPE-LR) described in all panels formulated by ethanol injection except panels (b) and (c) – formulated by extrusion. Scale bars: 200 nm and 100 nm for higher magnification insets for CryoTEM, 100 nm for 3D reconstruction, and 200 µm for dorsal zebrafish view.
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Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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