FIGURE

Fig. 7

ID
ZDB-FIG-230807-21
Publication
Elworthy et al., 2023 - Activated PI3K Delta Syndrome-1 mutations cause neutrophilia in zebrafish larvae
Other Figures
All Figure Page
Back to All Figure Page
Fig. 7

Tail-fin regeneration in pik3cdE525K/+ embryos. (A) Brightfield micrographs used to measure tail-fin regeneration for pik3cdE525K/+ embryos and their pik3cd+/+ siblings at 5 dpf, 2 days post injury (dpi). Top row shows uninjured controls. Transection was through the tip of the notochord. The regenerated area is illustrated with cyan false colour. Scale bar: 100 µm. (B) Chart of tail-fin regenerated area from micrographs as in A for 96 embryos. Bars show the mean±s.e.m., Mann–Whitney test. Data shown are from one experiment. A similar experiment using 5 dpf, 3 dpi larvae also detected no effect. (C) Chart of tail-fin regenerated area for pik3cdE1017K/+ embryos and their pik3cd+/+ siblings at 5 dpf, 2 dpi. Measured from micrographs of 96 embryos. Bars show the mean±s.e.m., Mann–Whitney test. Data shown are from one experiment. (D) Maximal-intensity projections of spinning disk confocal fluorescent micrograph stacks of 3 dpf TgBAC(il1b:EGFP)sh445;pik3cdE1017K/+ embryos and their pik3cd+/+ siblings, fixed and stained 3 h after tail transection. Fluorescence channels are shown for GFP and for endogenous peroxidase activity stain with Cy5-tyramide to identify neutrophils. Scale bar: 100 µm. (E) Chart of neutrophil TgBAC(il1b:EGFP)sh445 fluorescence intensity for single neutrophils at the injury site from each of 96 3 dpf sibling pik3cdE1017K/+ and pik3cd+/+ larvae, fixed and stained 3 h after tail transection as in D. Bars show the mean±s.e.m., Mann–Whitney test. Data shown are from one experiment.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.