FIGURE

Figure 2

ID

ZDB-FIG-230731-3

Publication

Sun et al., 2023 - lmo4a Contributes to Zebrafish Inner Ear and Vestibular Development via Regulation of the Bmp Pathway

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Figure 2

Morpholino-targeted knockdown and targeted knockout of lmo4a. (A) Bands of 483 bp and 324 bp represent correctly spliced and artificially mis-spliced lmo4a mRNA. Colored boxes indicate exons. Arrows signify primers used for RT-PCR and the black bar depicts lmo4a MO. (B) Protein alignment including the truncated protein generated by lmo4a MO. (C) RT-PCR of control embryos and those injected with 2 pg lmo4a MO showing effective but incomplete disruption of splicing (m, 100 bp ladder marker). (D–G) PCR and Sequencing analysis showing the lmo4a knockout efficiencies of the four gRNA targets.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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