Fig. 2

Fig. 2. TPhP exposure induced behavior changes of zebrafish larvae. Spontaneous movements (number of tail coiling per min) of zebrafish embryos under high (A) and normal (B) light intensity (2000/4400 Lux) at 21–24 hpf. Each treatment group consisted of 3 replicates and each replicate included 30 larvae. The velocity of zebrafish larvae following TPhP exposure was depicted by the panic and avoidance reflex for high-light stimulation (3-min dark, 1-min light and 3-min dark phases) (C), the alternating light/dark exploration model (5-min light and 5-min dark phases, repeated thrice) (D) and the light/dark flash model (5-s light and 5-s dark phases, eighteen cycles, total 180 s) (E) at 5 dpf. Each treatment group consisted of 3 replicates and each replicate included 32 larvae. All the data are presented as mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001, compared with the control. ### p < 0.001 compared to control (2000 Lux) within the same concentration (A/B).