FIGURE

Figure 3

ID

ZDB-FIG-230523-46

Publication

Silva et al., 2023 - In a zebrafish biomedical model of human Allan-Herndon-Dudley syndrome impaired MTH signaling leads to decreased neural cell diversity

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Figure 3

MCT8MO embryos have altered glial cell development during early neurogenesis. (A) Representative maximum intensity projection images of the spinal cord between somite 8-12 after glial cell labelling with ZRF-1 immunostaining (white, labelling GFAP fibers). In control embryos, at 15hpf glial cell fibers are organized in the developing ventral spinal cord; in MCT8MO embryos, the development of these cells is delayed, and only some scattered GFAP fibers are detected in the ventral-most neural tube. At 22hpf, the neural tube is closed, and glial cells can be detected throughout the spinal cord of CTRLMO and MCT8MO embryos; at 25hpf, the patterning of glial cells is altered in MCT8MO embryos. Red highlight - dorsal views, anterior spinal cord up. Blue highlight - lateral view, anterior spinal cord right. Green highlight - transversal view dorsal spinal cord up. All scale bars represent 25 µm. Dashed yellow lines denote spinal cord boundaries. (B) Quantification of the area of GFAP staining in a 2-myotome length of the spinal cord. n=9-17. CTRL (CTRLMO); MO (MCT8MO). Results are presented as mean ± SD; Statistical significance determined by t-test: two-sample, assuming equal variances: **p<0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)