FIGURE

Fig. 1

ID

ZDB-FIG-230501-168

Publication

Yang et al., 2022 - Heterogeneities of zebrafish vasculature development studied by a high throughput light-sheet flow imaging system

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Fig. 1

Principle of the high-throughput 3D imaging system, LS-FIS. (A) Sketch of the LS-FIS optics. L1-L3: lens; CL: cylinder lens; DM: dichroic mirror; M1-M3: mirror; O1-O2: objective; TL1-TL2: tube lens; CAM1-CAM2: camera; C: capillary. See Visualization 1. (B) Sketch of the LS-FIS fluidics. The fluidic flow was driven by a syringe pump between the buffer pool, sample reservoir, and dispense pool and controlled by two three-way valves and two photon-detectors. Val1-Val4: electromagnetic valve; LS: light sheet imaging; BF: bright filed imaging. See Visualization 2. (C) Timeline to reverse the pump, change the flow rate, open/close the valves with the signals from photon-detector PD1 (red) & PD2 (green), and the signal to trigger the imaging camera (blue). DET: detection of larva arrival; Trig: camera trigger. (D) Reconstruction of 3D embryo image by stacking light-sheet images with the correction of larva translational position.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biomed. Opt. Express