Fig. 7

Loss of lama1, lamb1a, and lamc1 results in abnormal basal accumulation of intracellular MRLC-GFP at the MHB. (A–E) Representative confocal images of WT-MRLC-GFP injected embryos imaged at 24 ss, WT (A), and mypt1 (B), lama1 (C), lamb1a (D), lamc1 (E) mutants. Yellow arrows represent directionality of intensity measurements that were taken from basal to apical. Light yellow region around arrow represents approximate 10 ​μm width included within measurements. (F–J) Normalized fluorescence intensity plot using quantification of average MRLC-GFP intensity from the basal edge to apical midline of the neuroepithelium at the MHB (blue and black lines). (G–J) Calculated p-values < ​0.05 were graphed and overlayed on the intensity plots to indicate regions of significant intensity differences between mutants and WT (red lines). (K–O) Basal-to-apical regions were generated based upon basal-to-apical distance creating five equal groups, 1–5. Average area under the curve was calculated from average intensity values for each region within all genotypes. (P) Fluorescence intensity plot of MRLC-GFP from basal to apical of all genotypes shown together (data from F-J). (Q) Average area under the curve of region 1, the most basal region, of all genotypes (data from K–O). At least three independent experiments are represented. n ​= ​6 embryos for WT, 7 embryos for mypt1 mutants, 12 embryos for lama1 mutants, 6 embryos for lamb1a mutants, and 8 for lamc1 mutants. Error bars in K–O represent standard deviation. Significance values ∗ P ​< ​0.05, ∗∗P ​< ​0.01, ∗∗∗P ​< ​0.005. White arrowheads indicate the MHBC. Scale bars: 20 ​μm.