FIGURE

Fig. 3

ID

ZDB-FIG-230103-3

Publication

Tyrkalska et al., 2022 - The Spike protein of SARS-CoV-2 signals via Tlr2 in zebrafish

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Fig. 3

Tlr2 mediates the S1WT-induced hyperinflammation in zebrafish. One-cell stage zebrafish eggs of Tg(lyz:dsRED2) (A), Tg(mfap4:mCherry) (B), Tg(NFkB-RE:eGFP) (C) and wild type (D, E) were microinjected with control or tlr2 crRNA/Cas9 complexes. At 2 dpf, recombinant S1WT (+) or vehicle (−) were injected in the hindbrain ventricle (HBV) of control and Tlr2-deficient larvae. Neutrophil (A) and macrophage (B) recruitment and number, and Nfkb activation (C) were analyzed at 6, 12 and 24 hpi by fluorescence microscopy, the transcript levels of the indicated genes were analyzed at 12 hpi by RT-qPCR (D), and caspase-1 activity was determined at 24 hpi using a fluorogenic substrate (E). Each dot represents one individual and the mean ± S.E.M. for each group is also shown. RT-qPCR data are depicted as a heat map in D with higher expression shown in darker color. P values were calculated using one-way ANOVA and Tukey multiple range test. ns, not significant, *≤p0.05, **p ≤ 0.01, ***p ≤ 0.001. auf, arbitrary units of fluorescence.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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