FIG 1

Lewis X (Le^x) and core fucose are expressed in 5-days post fertilization (dpf) zebrafish larvae. (A to C) Whole-mount immunohistochemistry staining in 5 dpf larvae of core fucose stained with fluorescein isothiocyanate (FITC)-labeled A. aurantia lectin (AAL, green) and counterstained with Hoechst 33342 (blue) at ×10 (A) and ×20 (B) magnification in the intestinal bulb and ×20 magnification along the posterior intestine (C). (D, E) Whole-mount immunohistochemistry staining in 5 dpf larvae of Le^X stained with anti-LeX primary antibody (green) and Hoechst 33342 (blue) at ×10 (D) and ×20 (E) magnification, representative image of Le^X staining along the intestinal tract. (F, G) Whole-mount immunohistochemistry staining of human norovirus (HuNoV)-infected zebrafish larvae at ×20 magnification costained with an anti-VP1 antibody (red) and FITC-AAL (F) or Le^X (G), with Hoechst 33342 as counterstaining. White arrows, colocalizing viral particles. (H) Tresholded Manders’ colocalization coefficient of HuNoV VP1 with fucose residues in the gastrointestinal tract of 5 dpf larvae (n = 5). Tresholded Manders’ coefficients are calculated with Imaris colocalization software. For panels A to E, adjacent tile pictures were merged together using the mosaic merge function of the LAS X software.