FIGURE

Fig. 6

ID
ZDB-FIG-220819-40
Publication
Morales et al., 2022 - Interleukin-10 regulates goblet cell numbers through Notch signaling in the developing zebrafish intestine
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Fig. 6

Enhanced Notch signaling and decreased goblet cell frequencies in mouse small intestine organoids treated with IL-10.

a Schematics for mouse SI organoid cultures. Organoids were grown in ENR media (containing EGF, Noggin, and R-Spondin) supplemented with recombinant murine IL-10 or BSA (Vehicle control). b RNA expression analysis by qRT-PCR from organoids treated with IL-10. Each dot represents SI organoids generated from an individual mouse (N = 5 independent mice). c Alcian blue staining of SI organoids in vehicle- and IL-10-treated organoids. Scale bar = 50 µm. d Percentage of the ab-stained area from the total organoid area. Dots represent single organoids imaged from 2 independent experiments/mice. e Flow cytometry analysis of organoids generated from SI crypts of Lgr5-GFP mice. Representative contour plots showing the percentage of intestinal stem cells (Epcam+ Lgr5-GFP+ cells) are shown. f Frequency of Lgr5+ intestinal stem cells (ISCs) after IL-10 treatments. Dots represent pools of organoids collected from 5 different mice. g Representative contour plots of WGA/CD24 stainings on disaggregated organoids treated with IL-10. h Frequencies of goblet cells (Epcam+ Lgr5-GFP- WGAhi CD24-) and paneth cells (Epcam+ Lgr5- WGAhi CD24+) from vehicle and IL-10-treated SI organoids. N = 5 independent mice. Independent experiments are represented by different symbol shapes in b, d, f and h. Conventional two-tailed t-test was performed in d, whereas paired two-tailed t tests were performed in b, f and h (*p < 0.05, **p < 0.01).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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