FIGURE

Fig. 4

ID

ZDB-FIG-220715-52

Publication

Berger et al., 2022 - Mob4-dependent STRIPAK involves the chaperonin TRiC to coordinate myofibril and microtubule network growth

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Fig. 4

Sarcomere organisation is compromised in mob4^geh mutants.

(A) Transmission electron micrograph depicted highly organised and arrayed myofibrils in 3-dpf-old siblings (n = 3). (B) Organised sarcomeres were rarely detected within mob4^geh homozygotes (n = 3). (B’) As shown in the magnification of the boxed area, sarcomeres were frequently disorganised and deposits of isolated filaments (asterisk) in addition to electron-dense structures (arrowhead), often associated with Z-disks, were found instead. (C) Electron-dense aggregates of mob4^geh homozygotes often showed a lattice structure (arrowhead) and (D) fragmented sarcomeres and widened Z-disks (double-arrowhead) were detected as well. (E) At 3 dpf, Gomori trichrome staining revealed subsarcolemmal dark blue structures within mob4^geh homozygotes but not siblings (n = 6 per genotype). Boxed areas are shown in higher magnification. (F) GFP fluorescence of transgenic ACTA1-GFP showed a striated pattern in 3-dpf-old siblings and a uniform pattern in mob4^geh homozygotes. Expression of ACTA1^D286G-GFP led to rod-shaped structures in siblings and exclusively amorphic aggregates within mob4^geh homozygotes (n = 6 per genotype). Scale bar sizes are indicated.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mob4^pc43/pc43
Observed In:	skeletal muscle cell sarcomere organization skeletal muscle cell skeletal muscle thin filament assembly skeletal muscle cell Z disc
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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