FIGURE

Fig. 2

ID
ZDB-FIG-220715-50
Publication
Berger et al., 2022 - Mob4-dependent STRIPAK involves the chaperonin TRiC to coordinate myofibril and microtubule network growth
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Fig. 2

The function of mob4 is lost within gemütlich mutants.

(A) Linkage analysis of gemütlich revealed a region of homozygosity on chromosome 9 with a peak between 32 to 33 Mb. (B) The MOB family member 4 (mob4) gene was located within the linked region. (C) Genomic sequences show that the mutant gemütlich harboured a mob4 allele with a premature stop codon in exon 2 (Q41X). (D) Western blot analysis using antibodies against human MOB4 showed epitope loss in mob4geh homozygotes. (E) Knockdown of mob4 by the morpholinos mob4_3D(+93–16) that targets the splice donor of exon 3 or (F) mob4_ATG(-9+16) that targets the translation start codon led to a reduction in birefringence. (G) Compared to control injected 3-dpf-old larvae (100 ± 1% and 100 ± 2%, respectively), administration of mob4_3D(+93–16) induced a reduction in birefringence to 71 ± 2% and mob4_ATG(-9+16) to 65 ± 6%. Crosses represent individual larvae (n = 6). (H) RT-PCR using primers targeting exons 1 and 5 of mob4 revealed altered splicing in mob4_3D(+93–16)-injected larvae. (I) The mob4-13 allele harboured a genomic deletion of 13 bp from exon 1 (g.5_17del). (J) Compared to 3-dpf-old siblings (both 100 ± 1%), the birefringence of mob4-13 homozygotes and mob4-13/geh compound heterozygotes was significantly reduced to 63 ± 1% and 61.1 ± 0.8%, respectively. Crosses represent averaged birefringence of clutches with a minimum of 6 larvae per genotype (n = 5 clutches). Data are presented as mean ± SEM; *** P < 0.001 calculated by Student’s t-test.

Expression Data
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Phenotype Detail
Acknowledgments
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