FIGURE

Fig. 3

ID

ZDB-FIG-220707-10

Publication

Zinani et al., 2022 - Gene copy number and negative feedback differentially regulate transcriptional variability of segmentation clock genes

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Fig. 3

Clock gene dosage increases correlated transcriptional variability more than uncorrelated one

(A) Reduced copy of her genes likely results in decreased burst sizes in her1^b567/ci301;her7^b567/hu2526 mutants compared to her1^ci301;her7^hu2526 mutants.

(B) One of the chromosomes has mutant her1 and her7 genes whereas the other has a large deletion including the her1-her7 locus in her1^b567/ci301;her7^b567/hu2526 mutants. Red stars (∗) mark point mutations causing premature stop codons.

(D) her1^b567/ci301;her7^b567/hu2526 embryos (n=12, N=2) have less her mRNA than double homozygous her1^ci301;her7^hu2526 embryos (n=17, N=2). The frequency histogram of total mutant her (her1+her7) RNA per cell is plotted in her1^ci301;her7^hu2526 (red), her1^b567/ci301;her7^b567/hu2526 mutant (blue) embryos.

(E and F) her1^b567/ci301;her7^b567/hu2526 embryos have both reduced correlated (E) and uncorrelated (F) transcriptional variability than her1^ci301;her7^hu2526. Error bars are two standard errors. n is the number of embryos; N is the number of independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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