FIGURE

Fig. 6

ID

ZDB-FIG-220628-64

Publication

Wang et al., 2022 - Zebrafish Xenograft Model for Studying Pancreatic Cancer-Instructed Innate Immune Microenvironment

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Fig. 6

Knockdown of REG4 in cancer cells suppresses tumor growth and activates innate immune response in zebrafish. (A) Relative expression of REG4 mRNA in PANC1, BxPC3, AsPC1, and CFPAC1 cells. (B) Western Blotting of REG4 and Actin in PANC1 and AsPC1 cells transfected with the control or REG4 siRNA. (C) The growth curve of in-vitro culture of PANC1 and AsPC1 cells transfected with the control or REG4 siRNA. (D) Representative confocal images of PANC1 and AsPC1 cells transfected with the control or REG4 siRNA in zebrafish xenografts at 4 dpi. (E) Relative tumor growth (4 dpi vs. 1 dpi) of the control tumor cells or cells with REG4 silencing. Results are shown as means ± SEM from nine different individuals (*** p < 0.001, t test). (F) Representative confocal images of ASPC1 cells in the control or REG4-silencing group in Tg(coro1a: GFP⁺) zebrafish. (G) Quantification coro1a: GFP⁺ innate immune cell percentage in AsPC1 tumor in the control and REG4-silencing groups. Results are from nine different individuals (* p < 0.05, t test). dpi, days post injection. Scale bars, 50 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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