FIGURE

Fig. 7

ID
ZDB-FIG-220628-46
Publication
Wiltbank et al., 2022 - Cd59 and inflammation regulate Schwann cell development
Other Figures
All Figure Page
Back to All Figure Page
Fig. 7

(A) Immunofluorescence (IF) showing Sox10-positive posterior lateral line nerve (pLLN) SCs (black/gray) at 55 hours post fertilization (hpf) in embryos treated with DMSO or 100 µM dexamethasone (Dex). Black dashed lines outline the pLLN. Sox10-positive pigment cells outside of the dashed lines (white asterisks) were not included in the analysis. (B) EdU incorporation assay showing sox10:nls-eos-positive, pLLN SCs (orange) pulsed with EdU (cyan) from 48 to 55 hpf in embryos treated with DMSO or 100 µM Dex. White dashed lines outline the pLLN. Magenta dashed lines outline neuromasts. (C) Scatter plot of the number of pLLN SCs at 55 hpf. Asterisks (*) indicate significant differences discovered with two-way ANOVA with Tukey’s post-hoc test using GraphPad Prism. Obelisk (†) indicates the significant difference discovered with Student’s t-test (GraphPad Prism) comparing WT DMSO and WT Dex alone (mean ± SEM: DMSO: WT: 12.0 ± 0.5, cd59uva48: 18.1 ± 0.6; Dex: WT: 10.4 ± 0.4, cd59uva48: 13.4 ± 0.6; two-way ANOVA p-values: WT DMSO vs. cd59uva48 DMSO: p<0.0001, WT Dex vs. cd59uva48 DMSO: p<0.0001, WT Dex vs. cd59uva48 Dex: p=0.0014, cd59uva48 DMSO vs. cd59uva48 Dex: p<0.0001; t-test p-value (compared WT only): WT DMSO vs. WT Dex: p=0.0206; dot = 1 fish). (D) Scatter plot of the number of EdU-positive SCs along the pLLN at 55 hpf in embryos treated with DMSO or 100 µM Dex (mean ± SEM: DMSO: WT: 4.6 ± 0.85, cd59uva48: 9.0 ± 0.68; Dex: WT: 2.7 ± 0.65, cd59uva48: 3.5 ± 0.53; p-values: WT DMSO vs. WT Dex: p=0.0091, WT DMSO vs. cd59uva48 DMSO: p=0.0266, WT Dex vs. cd59uva48 DMSO: p<0.0001, cd59uva48 DMSO vs. cd59uva48 Dex: p<0.0001; dot = 1 fish). These data were compared with Student’s t-test using GraphPad Prism. All data were normalized to units per 100 µm. All images were acquired with confocal imaging. Scale bars: (A, B) 25 µm.

Expression Data
Genes:
Antibody:
Fish:
Condition:
Anatomical Term:
Stage: Long-pec

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage: Long-pec

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife