Figure 2—figure supplement 1.

Ablation with nitroreductases (NTR)-metorodinazole (MTZ) system.

(A) Representative confocal stack images of 5 days post fertilization (dpf) TgBAC(pdgfrb:Gal4FF);Tg(14xUAS:3xFlag-NTR, NLS-mCherry);Tg(fli1:GFP) treated with 5 mM MTZ or vehicle for 16 hr. NTR expression was highly selective on mural cells (MCs) population. MCs were ablated after 16 hours of 5 mM MTZ treatment. Arrows indicate floorplate (FP) and hyperchord (HP) were not ablated by the treatment despite the expression of NTR. Scale bar: 100 μm. (B) Confocal stack images from Tg(fli1a:Myr-GFP) in control and ablated embryos described in Figure 2A post injection of Qtracker 705 vascular labels (shown in grey) into common cardinal vein. Arrows indicate lymphatic vessels labelled by leaked dye when injected or during the circulation in the control but not in the MC-ablated larva. Scale bar: 100 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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