Figure 1—figure supplement 1.

(A) Heatmap generated with confocal z-stack images from time-lapse images in the trunk of 2 days post fertilization (dpf) Tg(dab2:GALFF);Tg(UAS:GFP); Tg(pdgfrb:mCherry) embryos. Heatmap was generated based on the intensity of mCherry. Arrow indicates pdgfrb^high MC around aISV. (B) Confocal z-stack images from time-lapse images in the trunk of 2 dpf Tg(lyve1b: mCherry);Tg(kdrl:TagBFP);TgBAC(pdgfrb:GFP) embryo. Boxed regions are enlarged in the bottom. Arrowheads indicate pdgfrb⁺ MCs (green) around aISV (magenta) prior to (bottom left) and during (bottom middle and right) LEC (grey) migration. Scale bars: 50 or 30 μm (enlarged image). (C) Confocal z-stack images from time-lapse images in the trunk of Tg(dab2:GALFF);Tg(UAS:GFP) (grey);Tg(pdgfrb:mCherry) (green) in left panels and TgBAC(pdgfrb:GAL4FF);(UAS:GFP) in right panels (green) and Tg(dab2:GALFF);Tg(UAS:GFP) (grey) in middle panels. White arrow heads, MCs appear around aISV. Arrow, sprouting front of migrating LEC. Scale bar: 100 μm. (D) Quantification of MC (n = 30) movement from n = 7 embryos as still (n = 26) and mobile (n = 4) during the time-lapse videos as in (Figure 1E). Data are presented as ratio of total number of cells counted.