FIGURE

Fig. 2.

ID

ZDB-FIG-220316-60

Publication

Lanham et al., 2022 - Genetically inducible and reversible zebrafish model of systemic inflammation

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Fig. 2.

GSP-Il-1β^mat driven systemic inflammation is dose dependent. (A) Dose-dependent effects of Teb on neutrophil numbers following 2 days of exposure to either vehicle (DMSO) or increasing concentrations of Teb (n=6 for each concentration, one way ANOVA with Tukey HSD). (B) RT-PCR of GSP-Il-1β^mat and actb1 (Actin) at 4 dpf following exposure of embryos to vehicle or the indicated concentration of Teb at 2 dpf. Amplification of linearized plasmid DNA (25 pg) was included as a positive control. (C) Embryos were exposed to vehicle or indicated concentrations of Teb at 2 dpf (red arrowhead) and counted daily for 10 days to obtain Kaplan–Meier survival curves for each cohort, (n=40 for each concentration). (D) Representative images of larvae from (A) at indicated concentrations of tebufenozide. Brightfield images are minimum intensity projections, and mCherry images are maximum intensity projections of confocal stacks acquired for neutrophil counts. For all figures data are shown as the mean±s.e.m. (*P<0.05, **P<0.01, ***P<0.001).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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