Fig. 4

(A) Comparison of oxygen uptake between lepr−/− and wt larvae at 28 °C from 5 to 19 dpf (exogenous feeding period). Each time-point is the mean of 6 vials, each containing 8–10 larvae (±SE). (B) Comparison of oxygen uptake/wet weight between lepr−/− and wt juveniles at 28 °C from 26 to 40 dpf (exogenous feeding period). Each time-point is the mean of 15 vials, each containing 1 juvenile (±SE). The number of fish per vial was chosen according to age and size. Two-way ANOVA shows significant effects of developmental age [F (2, 29) = 66.45; p < 0.0001] and genotype [F (1, 29) = 7.606; p < 0.01] for oxygen uptake in the 5–19 dpf range and significant effects of developmental age [F (2, 79) = 4.27; p < 0.05] and genotype [F (1, 79) = 16.29; p < 0.0001] for oxygen uptake/wet weight in the 26–40 dpf range. A post-hoc Turkey’s multiple comparison was used to assess for specific pairwise differences (*** = p < 0.001). For detailed information see Supplementary Table 2.