FIGURE

Fig. 2

ID

ZDB-FIG-220306-2

Publication

Elsaid et al., 2022 - Reduced α-galactosidase A activity in zebrafish (Danio rerio) mirrors distinct features of Fabry nephropathy phenotype

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Kidney tissue distribution and enzyme activity in wild type zebrafish. (A) Immunohistochemistry (IHC) staining for Zebrafish α-GAL in wild-type adult zebrafish kidney. The expression pattern is similar in both genders. The protein was abundantly detectable in the cytoplasm of renal tubule cells, and to a lower extent in the glomerulus. Hematoxylin was used as counterstain. The lower panel shows the three renal structures, as stained with Periodic Acid Schiff (PAS). Scale bar (in black) = 50um. (B) Wild-type zebrafish α-GAL activity as evaluated in the whole embryo, larvae, and juvenile whole body tissue lysates and in adult male and female kidney tissue lysates (Kruskal-Wallis, P ≤ 0.05). Results of the Dunn post hoc test show a significant difference (*) between juvenile and larvae (P < 0.05) and juvenile and adult female specimens (P < 0.05). The violin plot represents these results within the 95th percentile. G = glomerulus, PT = proximal tubule, DT = distal tubule. Validation of the antibody is provided in Sup.4.

Expression Data

Gene:	gla
Antibody:	Ab1-gla
Fish:	AB/TU
Anatomical Terms:	renal glomerulus renal tubule cytoplasm
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol Genet Metab Rep