FIGURE

FIGURE 5

ID

ZDB-FIG-220131-121

Publication

Hopfenmüller et al., 2022 - The Wilms Tumor Gene wt1a Contributes to Blood-Cerebrospinal Fluid Barrier Function in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 5

Wt1a inactivation reduces the blood-CSF barrier function. Fluorescently labeled dextrans were injected into the cardinal vein of 5 days old larvae of the wt1a ^ex1_del5 line and leakage into the ventricles was analyzed. (A) Representative overlay images of transmission and rhodamin-dextran (10 KDa) fluorescence recorded by light sheet microscopy of larvae that were fixed 4 h after injection. The dotted line marks the ventricular system. (B) Ratios of fluorescence intensities of rhodamin-dextran (10 KDa) and fluorescein-dextran (40 kDa) in the ventriclular region relative to the respective fluorescence in the pupil were estimated in vivo 40 min post injections. n = 24 for wild types and 22 for mutants; p-values according to ANOVA: 0.003 and 0.002 (**) for 10 and 40 kDa, respectively.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol