Fig. 3

Summary of peak fluorescence intensity and functional connectivity data from vehicle (water) control larvae tested across 65 experimental datasets. Panel (a) shows the peak fluorescence intensity data per brain region of interest (ROI) as the average baseline-corrected fluorescence intensity (Δf/f = (f1 − f0)/f0 * 100, where f1 = peak fluorescence intensity and f0 = baseline fluorescence intensity within each ROI) of larvae within each control group (per column), presented as the % of the maximum peak intensity shown within that experimental dataset (n = 7–9 larvae per group). The most active brain region in each dataset is represented by 100%, with decreasing red/orange colour intensity representing ROIs with lower levels of comparative activity. Note the high consistency across experiments in brain regions exhibiting elevated basal activity, especially those that showed a mean peak intensity of >33% higher basal activity than the overall mean across all regions: intermediate hypothalamus, area postrema, inferior olive, locus coeruleus, Mauthner cells, medial vestibular nucleus, inferior raphe, spinal cord and spinal cord neuropil. Panel (b) shows the resting state functional connectome of control larvae presented as the average across 65 experimental datasets along with a table summarising the 10 most connected brain regions and the 10 strongest functional connections (n = 516 larvae). Abbreviated brain regions are as follows: area postrema (AP); cerebellum (C); corpus cerebelli (CC); dorsal thalamus (DT); eyes (E); eminentia granularis (EG); eminentia thalami (ET); habenulae (H); intermediate hypothalamus (I); inferior olive (IO); interpeduncular nucleus (IPN); lobus caudalis cerebelli (LC); locus coeruleus (Lco); lateral reticular nucleus (LRN); Mauthner (Mt); medial vestibular nucleus (MVN); noradrenergic neurons of the interfascicular and vagal areas (NIV); spinal cord neuropil (Np); olfactory bulb (OB); olfactory epithelium (OE); pineal (P); preoptic area (PA); pallium (pa); pretectum (PT); raphe inferior (RI); rostral hypothalamus (Ro); raphe superior (RS); spinal cord (SC); subpallium (Sp); posterior tuberculum (TB); torus longitudinaris (TL); tegmentum (tm); tectum neurophil (TN); torus semicircularis (TSC); tectum stratum periventriculare (TSP); tangential vestibular nucleus (TVN); Valvula cerebelli (V); vagal ganglia (VG); ventral thalamus (VT). Note: Positions of regions approximate to allow spacing of nodes and visualisation of connections