Fig. 6

Dll4 and Notch-intracellular domain alters collagen IV secretion and gene expression. (a) Representative images of ECs plated on coverslips coated with recombinant protein delta like ligand 4 (Dll4) (1ug/ml). (b) Graph of collagen IV (Col IV) extracellular ratio in ECs plated on Dll4 coated coverslips compared to culture media controls. N number of cells. (c) Graph of relative Hes1 gene expression in ECs cultured in VEGF-containing, serum-starve (SS) media or plated on Dll4 ligand. N number of replicates. (d) Graph of relative DENNd4C gene expression in ECs cultured in either VEGF-containing, SS media or plated on Dll4. N number of replicates. (e) Representative images of ECs expressing Notch1 intracellular domain (NICD) cultured in VEGF-containing media. N number of replicates. (f) Graph of Col IV extracellular ratio in ECs between indicated groups. N number of cells. (g) Graph of relative Hes1 gene expression in ECs between indicated groups. N number of replicates. (h) Graph of relative DENNd4C gene expression in ECs between indicated groups. N number of replicates. (i) Representative images of ECs stained for Col IV (green), actin (grey), DNA (blue) and flag (light blue) cultured with VEGF, in SS media, in SS media with DAPT, or in SS media with DAPT overexpressing DENNd4C-flag. (j) Graph of Col IV extracellular ratio in indicated groups. N number of cells. For all experiments, data represented as mean ± 95% confidence intervals. Black bars indicate comparison groups with indicated p-values. All p-values are from two-tailed Student’s t-test from duplicate experiments. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001; ns not significant