FIGURE

FIGURE 2

ID

ZDB-FIG-210725-2

Publication

Shanaka et al., 2021 - Growth and Antioxidant-Related Effects of the Reestablished Ascorbic Acid Pathway in Zebrafish (Danio rerio) by Genomic Integration of L-Gulonolactone Oxidase From Cloudy Catshark (Scyliorhinus torazame)

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FIGURE 2

Selection of Tg fish expressing b-actin:sGULO:mCherry. (A) A schematic of the b-actin:sGULO:mCherry expression construct. The β-actin promoter sequence was used to drive the expression of sGULO fused with mCherry, which acts as a reporter for construct expression in Tg zebrafish. The poly-A segment includes a -G cap mRNA followed by Tol2 sequences, which act as transposable segment sequences needed to integrate the construct into the zebrafish genome. (B) The mCherry fluorescent signal in Tg and Wt fish. (C) Positive Tg embryos showed red fluorescence in their muscles, and negative fish lacked this fluorescent signal. The positive and negative F0 generation embryos were anesthetized using tricaine, mounted on a microscope slide using 2% methylcellulose, and observed under a fluorescent microscope (Leica, DM600B) under ×100 and ×200 magnifications.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.