ZFIN ID: ZDB-FIG-210715-6
Chambers et al., 2020 - Ppargc1a Controls Ciliated Cell Development by Regulating Prostaglandin Biosynthesis. Cell Reports   33:108370 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 5 ptgs1 Functions Downstream of ppargc1a during Ciliogenesis

(A) Schematic depicting several Ppargc1a consensus sites located within 1.5 kb of the ptgs1 open reading frame (ORF).

(B) Twenty-four hours post-fertilization WT (top) and ppargc1asa13186/sa13186 (bottom) zebrafish stained via WISH for ptgs1 (n > 60). Scale bar, 100 μm.

(C) Relative ptgs1 mRNA expression in WT and ppargc1a morphants.

(D) Whole-mount immunofluorescence for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI in the proximal pronephros of WT (top), ppargc1a morphants (middle), and ppargc1a morphants injected with ptgs1 cRNA (bottom). Scale bar, 7 μm.

(E) Cilia length for the proximal pronephros treatment groups shown in (D).

(F) Graph of the percentage of ciliated basal bodies/total basal bodies per 100 μm in the proximal pronephros.

(G) Whole-mount immunofluorescence for acetylated α-tubulin (cilia, green), γ-tubulin (basal bodies, red), and DAPI in the distal pronephros of WT (top), ppargc1a morphants (middle), and ppargc1a morphants injected with ptgs1 cRNA (bottom). Scale bar, 7 μm.

(H) Cilia length for distal pronephros treatment groups shown in (G).

(I) Graph of the percentage of ciliated basal bodies/total basal bodies per 100 μm in the distal pronephros.

(J) WISH for the MCC marker odf3b in WT (top), ppargc1a morphants, and ppargc1a morphants injected with ptgs1 cRNA (bottom). Scale bar, 90 μm.

(K) Number of MCCs per nephron.

(L) Proposed mechanism by which ppargc1a regulates MCC development and ciliogenesis via PGE2 levels by controlling ptgs1 expression.

Data are represented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 (one-way ANOVA or t test); n.s., not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ptgs1 TU standard conditions Prim-5 pronephros distal region ISH
ppargc1asa13186/sa13186(TU) standard conditions Prim-5 pronephros distal region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ppargc1asa13186/sa13186(TU) standard conditions Prim-5 pronephros distal region ptgs1 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.