Figure 4

Effects over gene and enzyme activity levels in whole body of 8 dpf old zebrafish larvae following pharmacological modulation of the serotonergic system. (A) Expression of tph1α involved in serotonin synthesis process; (B,C) Expression of mao and MAO activity, respectively, involved in the degradation of serotonin; (D) Expression of sert and vmat2 genes that respectively regulate serotonin transport from the synaptic cleft back to the presynaptic neuron and the transport of serotonin form the cell cortisol into synaptic vesicles. Data are from 2 independent experiments and are reported as scatter plots with the median (red line) and n = 6–8 for all treatment groups. Significance was set to p < 0.05 and can be represented as * when p < 0.05; ** when p < 0.01 and *** when p < 0.001, one-way ANOVA with Dunnett’s multiple comparison test.