FIGURE

Figure 8

ID
ZDB-FIG-210617-62
Publication
Ceriani et al., 2021 - Gonadotropin Releasing Hormone (Gnrh) Triggers Neurogenesis in the Hypothalamus of Adult Zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Figure 8

BrdU labeling reveals GnRH-induced increases in cells in the POA. (A–E,A’–E’) Quantification of the effects of GnRH (1 µM GnRH) on the POA of adult zebrafish: Cells were counted in transverse 20 µm cryosections of the POA (12 sections). (A–E) GnRH control. (A–E’) GnRH treatment (1 µM GnRH). (F) Control with Sox2+ cells (arrow), cytoplasmic Sox2+ (arrowhead) (F’) GnRH treatment increased cytoplasmic Sox2+ cells in the PPp1 (arrowheads). (G) Significant increases in BrdU labeled cells in the GnRH-treated animals. (H) Significant increases in cytoplasmic Sox2+ cells increased in the GnRH-treated animals. *** p < 0.001; ** p < 0.01; Student’s t-test, SEM. (I,J) Number of cells located in the regions of the POA scored in transverse cryosections (20 µm: PPa1 = 2, PPa2 = 2, PPa3 = 3, PPa4 = 4 and PPp1 = 2). (I) Number of BrdU+ cells (J) number of cytoplasmic Sox2+ cells in GnRH-treated animals. * p < 0.05, Student’s t-test, SEM. (K) GnRH treatment increased the number of BrdU+ cells (blue arrow) and cytoplasmic Sox2+ (blue arrow) cells, where few cytoplasmic Sox2+ were also BrdU+. (A–E,A’–E’): Sections labeled with DAPI (blue). Scale bars: 30 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.