Figure 2

Cells lining the diencephalic ventricle (DiV) express neural progenitor markers. (A–J) Expression of Vim, Zrf1, and Sox2 in transverse paraffin-sections of 10 μm of the POA. (A–E) Cells immuno-positive for anti-Vim (red) and anti-Zrf1 (green). (A,B) Vim+/Zrf1+ positive cells are distributed dorsomedially in the wall of the third ventricle (boxed area), and signals are co-localized ((A’,B’), arrows). (C) Fewer Vim+/Zrf1+ cells are observed in PPa3 (boxed area) with limited co-localization ((C’), arrow). ((A–C), asterisks) Vim+/Zrf1+ cells are located in the ventrolateral region with long processes that extend towards the DiV (arrowheads). (D) Vim-/Zrf1- cells with fusiform nuclei are found in at ventral sur-face of POA ((D’), asterisk). (E) Vim+ and Zrf1+ label in dorsomedial region (boxed area), is co-localized ((E’), arrow). (F–J) Cells immune-positive for anti-Vim and anti-Sox2. (F–H) Vim+ cells also label with anti-Sox2 (red) (boxed area), and Sox2+ cells are observed in dorsal-ventral regions of the ventricle wall. (F’–H’) Higher magnification views of box areas in F-H: Vim+/Sox2+ co-localization in cells (arrows). (I) Cells with fusiform nuclei are Sox2+ and are located in the ventral wall ((I’), arrow). (J) Vim+ and Sox2+ boxed area, co-localize in ventricle wall ((J’), arrow). Diencephalic ventricle (DiV); magnocellular nucleus (PM); optic nerve (ON); anterior part of the parvocellular preoptic nucleus (PPa); posterior part parvocellular nucleus (PPp); suprachiasmatic nucleus (SC). All sections labeled with DAPI (blue). Scale bar: 30 μm.