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ZDB-FIG-210519-16

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Zhang et al., 2021 - Rational construction of a reversible arylazo-based NIR probe for cycling hypoxia imaging in vivo

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Fig. 1

Reversible imaging mechanism and behavior of HDSF.

a Normal azobenzene-derived fluorescent probes for hypoxia and their reductive decomposition of N–N bond by the hypoxic microenvironment in living systems. b The reversible hypoxia sensing mechanism of probe HDSF. The square and fluorophore in black represent the quenched-fluorophore, and those in bright magenta represent the emitting-fluorophore. c Fluorescent spectra of 20 μM HDSF in PBS buffer (0.1 M, pH 7.4, 2% DMSO, v/v) containing rat liver microsomes (RLM, 250 μg mL⁻¹) and NADPH (100 μM) recorded in normoxia-hypoxia cycles. d Fluorescence intensity of HDSF at 705 nm detected in (c). e Fluorescent spectra of 20 μM HDMA in PBS buffer (0.1 M, pH 7.4, 2% DMSO, v/v) containing RLM (250 μg mL⁻¹) and NADPH (100 μM) recorded in normoxia-hypoxia cycles. f Fluorescence intensity of HDMA at 709 nm detected in (e). Ctrl: PBS buffer with RLM and NADPH. λ_ex, 650 nm.

Expression Data

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Antibody Labeling

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