FIGURE

Figure 1—figure supplement 1.

ID

ZDB-FIG-210514-27

Publication

Yi et al., 2021 - A calcineurin-mediated scaling mechanism that controls a K⁺-leak channel to regulate morphogen and growth factor transcription

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Figure 1—figure supplement 1.

Induction of developmental genes after induction of <italic>kcnk5b</italic> expression.

(A) qRT-PCR results of shh and lef1 expression from caudal fins of 6-month-old zebrafish comparing before (-HS) and 6 hr after (+HS) heat shock induction of the Tg[hsp70:kcnk5b-GFP] transgene in the caudal fins. (B) Confocal image of immunohistochemistry staining for GFP expression in a cross-section of the distal tip of the adult fin after heat shock induction of kcnk5b-GFP in a fish harboring the Tg[hsp70:kcnk5b-GFP] transgene. The lower left inset is an enlarged view of the expression of the transgene in the fin. (C) Confocal image of DAPI-stained nuclei of the same confocal section in panel A. (D) Overlay of the confocal GFP and DAPI images. (E) qRT-PCR results for the indicated genes in the caudal fin from AB non-transgenic and Tg[hsp70:knck5b-GFP] fish after daily heat-shock pulses over 3 days. (F) qRT-PCR for GFP expression in AB wild-type strain fish and kcnk5b-GFP fish after daily heat-shock pulses over 3 days. The data for each panel represents three or more experiments. The graphed data points show all technical replicates.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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