FIGURE

Figure 4

ID

ZDB-FIG-210503-93

Publication

Ko et al., 2021 - Differential Regulation of Ca²⁺-Activated Cl^- Channel TMEM16A Splice Variants by Membrane PI(4,5)P₂

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Figure 4

Specific TMEM16A current inhibition via the depletion of PI(4,5)P2 but not PI4P or PI(3,4,5)P3. (A) Strategy used to dephosphorylate PI4P, PI(4,5)P₂, or PI(3,4,5)P₃ using a rapamycin-induced dimerization system. RF-Sac1, 4-phosphatase; RF-INPP5E, 5-phosphatase; CF-PTEN, PI(3,4,5)P₃ phosphatase. CF was constructed without a phosphatase enzyme. (B) Representative currents before (black trace) and after (colored trace) the addition of rapamycin (1 μM) for 1 min in control cells co-expressing RF-Sac1, RF-INPP5E, CF, or CF-PTEN. (C) Summary of inhibition (%) in (B) by rapamycin-induced translocation of RF-Sac1, RF-INPP5E, CF, or CF-PTEN to the plasma membrane for TMEM16A(ac). Top: RF-Sac1, n = 4; RF-INPP5E, n = 7. Bottom: CF, n = 4; CF-PTEN, n = 4. Dots indicate the individual data points for each cell. Bars indicate means ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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