FIGURE

Fig. 2

ID

ZDB-FIG-201126-2

Publication

Farooq et al., 2020 - RRP7A links primary microcephaly to dysfunction of ribosome biogenesis, resorption of primary cilia, and neurogenesis

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Fig. 2

RRP7A is expressed in RGCs and cilia in the developing human neocortex aged 19 wpc.

a, b DAB staining depicting the expression of RRP7A in the human parietal cortex in low a and higher b magnification of the ventricular zone (VZ). Scale bars, 5 mm a and 0.5 mm b. c, d Higher magnification of zones #1 and #2 boxed in b. RRP7A reactivity is high in cilia (open arrows) c and in RGCs (closed arrows) d. Scale bars, 40 µm. e IFM analysis of the region depicted in b showing expression of RRP7A (red) in RGCs marked with Vimentin (green) and localization to cilia (open arrows). Scale bar, 50 µm. Insert: shifted overlay of region boxed in the merged panel showing RRP7A expression in RGCs (closed arrow). Insert scale bar, 10 µm. f DAB staining depicting the expression of RRP7A in RGCs in a section of the temporal cortex and hippocampal formation. Scale bar, 5 mm. g, h Higher magnifications of the zone boxed #1 in f showing expression of RRP7A at the cortical plate (CP). Scale bar, 0.2 mm. h Higher magnification of the zone boxed in g showing expression of RRP7A in CP RGCs (closed arrows). Scale bar, 50 µm. i Higher magnification of the zone boxed #2 in f showing expression of RRP7A (left panel) in RGCs at the outer surface along the hippocampus (F: Fimbria). RGCs (closed arrow) in this region are further marked with Glial Fibrillary Acidic Protein (GFAP) (right panel). Scale bars, 0.2 mm. Low magnification with GFAP staining is also shown in Supplementary Fig. 2a. j IFM analysis of the area of fimbriae shown in (i) presenting expression of RRP7A (red) in RGCs marked with Vimentin (green). Scale bar, 0.1 mm. k Higher magnifications of the zone boxed in j showing expression of RRP7A in RGCs (closed arrow). Scale bar, 25 µm. IZ/OFL Intermediate zone/outer fiber layer.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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