FIGURE

Fig. 4

ID

ZDB-FIG-201009-4

Publication

Chakraborty et al., 2020 - Converting lateral scanning into axial focusing to speed up three-dimensional microscopy

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Accelerating axially swept light-sheet microscopy (ASLM).

a Schematic principle of ASLM: a thin light sheet is scanned in its propagation direction, and only the region within the beam waist (red and green bars) is read out by an sCMOS camera. b Point spread function in the XZ plane. c, d RPE cells labelled with GFP-vimentin, imaged with ASLM with a 50 ms integration time. XZ and YZ maximum intensity projections are shown. e, f The same cell imaged with ASLM at 5 ms integration time. XZ and YZ maximum intensity projections are shown. g Genetically encoded multimeric nanoparticles (GEMs) inside two MV3 cells, as imaged by ASLM over a 20 ms image integration time, and 3.57 volumes per second. h, i Axial YZ view of the perinuclear region at two timepoints. Yellow circles indicate detected vesicles, and blue lines illustrate cumulative tracks. Scale bars: b, h, and i 1 µm; d and g 10 µm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Light Sci Appl