FIGURE

Fig. 2

ID

ZDB-FIG-201009-2

Publication

Chakraborty et al., 2020 - Converting lateral scanning into axial focusing to speed up three-dimensional microscopy

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Remote focusing using a remote step mirror and a planar mirror inclined by 7.5°.

a PSFs obtained using a remote mirror with three steps. b PSFs generated from discrete lateral scanning steps over a planar, tilted mirror. Green arrows depict the zoomed-in view. c Plot showing the full width at half maximum (FWHM) of the PSFs at different axial focus positions. The FWHM of the PSF calculated in the X-direction (red) and Y-direction (black). d Continuous axial scanning obtained by driving the GSM with a triangular waveform at 100 Hz using a tilted, planar mirror. Scale bar: a–d 10 µm. The scale bar in the zoomed-in image of b is 1 µm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Light Sci Appl