ZFIN ID: ZDB-FIG-200714-7
Borini Etichetti et al., 2020 - Expression of zebrafish cpsf6 in embryogenesis and role of protein domains on subcellular localization. Gene expression patterns : GEP   36:119114 Full text @ Gene Expr. Patterns
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage Range: 2-cell to Protruding-mouth

Fig. 2 Analysis of cpsf6 expression during zebrafish development. a) cpsf6 expression levels were monitored by RT-qPCR on cDNA from embryos of the indicated stages: 1–2 cell, 1 k-cell, 60% epiboly, 21 somites, prim-5, prim-16, long-pec and protruding-mouth. Data was normalized using gapdh mRNA levels as internal control and is shown as relative abundance comparing with 1–2 cell stage. b) Analysis of cpsf6 mRNA levels by semi quantitative RT-PCR on 1–2 cell, 1 k-cell, 20 somites and prim-5 stages. As an internal control, ef1α mRNA levels were determined on the same RNA samples. c) cpsf6 mRNA distribution in embryos at 60% epiboly and prim-5 stages analysed by whole mount in situ hybridization (scale bars: 200 μm). S: sense RNA probe (negative control), AS: antisense probe. d) Western blot analysis on extracts from 1 k-cell, 20 somites, prim-5, long-pec and protruding-mouth stages using CPSF6 antibody.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cpsf6 AB standard conditions 2-cell whole organism RTPCR
1-cell whole organism RTPCR
1k-cell whole organism WB
Shield whole organism ISH
Shield whole organism RTPCR
20-25 somites whole organism RTPCR
Prim-5 whole organism ISH
Prim-5 whole organism RTPCR
Prim-15 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Gene expression patterns : GEP for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene expression patterns : GEP, 36, Borini Etichetti, C.M., Tenaglia, A., Arroyo, M.N., Girardini, J.E., Expression of zebrafish cpsf6 in embryogenesis and role of protein domains on subcellular localization, 119114, Copyright (2020) with permission from Elsevier. Full text @ Gene Expr. Patterns