FIGURE

Fig. 2

ID

ZDB-FIG-200614-25

Publication

Tie et al., 2019 - Interleukin-6 signaling regulates hematopoietic stem cell emergence

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Il6 signaling is required for HSC-independent neutrophil development, but is not indispensable for the development of HSC-independent macrophages and primitive erythrocytes.

a Representative images of Std, Il6r, and Il6 morphants detected by WISH for mpx and l-plastin expression at 28 hpf. The white block denotes the posterior blood island (PBI) region, and the black block denotes the PBI and trunk regions. b Qualitative phenotype distribution of embryos from a scored with medium (normal) and low (down) mpx and l-plastin expression, respectively. Medium, white bar; low, gray bar. c Confocal tracking of HSC-independent macrophages in mpeg1:GFP transgenic embryos injected with Std, Il6r, and Il6 MOs at 32–36 hpf. d Enumeration of mpeg1⁺ macrophages cells in the yolk sac, PBI, and trunk regions from c. Bars represent the means ± SEM of Std (n = 12), Il6r (n = 12), and Il6 (n = 12) morphants. n.s., Not significant. e Representative images of Std, Il6r, and Il6 morphants interrogated by WISH for gata1a expression at 24 hpf. f Qualitative phenotype distribution of embryos from e scored as in b

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Exp. Mol. Med.