Figure 3

Identification of residues involved in homodimer interaction interfaces. Contact maps of inter-chain residue interactions of the mouse (a) and zebrafish (b) PATZ1 BTB domain crystal structures. Hydrophobic interactions (green), hydrogen bonds (blue) and ionic bonds (red) are indicated. The relevant elements of the secondary structures are shown for orientation. A highly charged dimerization interface mediates PATZ1 BTB homodimerization. (c) A split homodimer view in surface representation and completed with the modelled loop highlights the residues involved in the interaction interface; positively (blue) and negatively (red) charged residues are annotated and neutral residues are shown in purple. Inter-chain salt bridges that persist above the threshold are indicated by straight lines and those that do not persist by dotted lines. (d) The zebrafish PATZ1 dimerization interface is also shown as a split homodimer view for comparison. Numbering refers to the crystal structure files.