PUBLICATION
Structural analysis of the PATZ1 BTB domain homodimer
- Authors
- Piepoli, S., Alt, A.O., Atilgan, C., Mancini, E.J., Erman, B.
- ID
- ZDB-PUB-200606-10
- Date
- 2020
- Source
- Acta crystallographica. Section D, Structural biology 76: 581-593 (Journal)
- Registered Authors
- Keywords
- BTB, PATZ1, POZ, co-repressors, dimerization interface, structure dynamics, transcription factors
- MeSH Terms
-
- Repressor Proteins/chemistry*
- Zebrafish Proteins/chemistry*
- Zebrafish/metabolism
- Neoplasm Proteins/chemistry*
- Animals
- Mice
- Protein Multimerization
- BTB-POZ Domain*
- PubMed
- 32496219 Full text @ Acta Crystallogr D Struct Biol
Citation
Piepoli, S., Alt, A.O., Atilgan, C., Mancini, E.J., Erman, B. (2020) Structural analysis of the PATZ1 BTB domain homodimer. Acta crystallographica. Section D, Structural biology. 76:581-593.
Abstract
PATZ1 is a ubiquitously expressed transcriptional repressor belonging to the ZBTB family that is functionally expressed in T lymphocytes. PATZ1 targets the CD8 gene in lymphocyte development and interacts with the p53 protein to control genes that are important in proliferation and in the DNA-damage response. PATZ1 exerts its activity through an N-terminal BTB domain that mediates dimerization and co-repressor interactions and a C-terminal zinc-finger motif-containing domain that mediates DNA binding. Here, the crystal structures of the murine and zebrafish PATZ1 BTB domains are reported at 2.3 and 1.8 Å resolution, respectively. The structures revealed that the PATZ1 BTB domain forms a stable homodimer with a lateral surface groove, as in other ZBTB structures. Analysis of the lateral groove revealed a large acidic patch in this region, which contrasts with the previously resolved basic co-repressor binding interface of BCL6. A large 30-amino-acid glycine- and alanine-rich central loop, which is unique to mammalian PATZ1 amongst all ZBTB proteins, could not be resolved, probably owing to its flexibility. Molecular-dynamics simulations suggest a contribution of this loop to modulation of the mammalian BTB dimerization interface.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping