ZFIN ID: ZDB-FIG-200515-21
Wang et al., 2020 - The micropeptide LEMP plays an evolutionarily conserved role in myogenesis. Cell Death & Disease   11:357 Full text @ Cell Death Dis.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 2-cell to 14-19 somites

Fig. 5 a In situ hybridization to examine the localization of MyolncR4 at different developmental stages of zebrafish embryos with a specific probe targeting zLEMP. hpf, hours post fertilization. Scale bar, 200 μm. b Diagram to show the targeting sequence of the LEMP-AMO. c Representative images of zebrafish at 24 or 48-hpf after injection of different amounts of LEMP-AMO at one-cell stage. Scale bar, 200 μm or 25 μm. d Representative images of zebrafish at 24 or 48-hpf after injection of LEMP-AMO in the presence or absence of the zebrafish LEMP mRNA at one-cell stage. Scale bar, 200 or 25 μm. e MHC immunostaining to examine myofiber alignment in 48-hpf embryos injected with Cntl-AMO, LEMP-AMO, LEMP-AMO with zLEMP mRNA, or LEMP-AMO with mLEMP mRNA, respectively. Scale bar, 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lnc.lemp TU standard conditions 2-cell whole organism ISH
Shield whole organism ISH
14-19 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Cell Death & Disease for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Death Dis.