ZFIN ID: ZDB-FIG-200426-20
Zhang et al., 2020 - Wnt-PLC-IP3-Connexin-Ca2+ axis maintains ependymal motile cilia in zebrafish spinal cord. Nature communications   11:1860 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage Range: Long-pec to Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Adult

Fig. 5 c<italic>x43</italic> null zebrafish have reduced motile cilia in ECs.

a Electropherograms of the target sequences of the cx43 gDNA in WT and cx43−/− zebrafish. Arrow indicates the deletion of the T nucleotide. b Zebrafish (n = 71) at 2 months post-fertilization (mpf) from mating of cx43+/− zebrafish were genotyped for cx43. c Images of 3 mpf zebrafish with the indicated cx43 genotype. d WT or cx43−/− embryos at 2 dpf were immunostained with anti-acetylated-α-tubulin antibody, imaged with a confocal microscope and genotyped for cx43. Arrowheads represent motile cilia. Dorsal view anterior to the left. Scale bar = 20 μm. e Larvae at 8 dpf from mating of cx43+/− zebrafish were cut into cranial and caudal halves. The cranial half was used for cx43 genotyping, and the caudal half was coronally sectioned at a thickness of 14 μm and then processed for IF staining with anti-acetylated-α-tubulin antibody. Arrowheads represent motile cilia. Dorsal view anterior to the left. Scale bar = 30 μm. f Quantification of the number of cilia per frame in embryos in e. Mean ± SD. ****P < 0.0001 by one-way ANOVA with Tukey’s HSD post hoc test (cx43+/+ = 7 embryos; cx43+/− = 7 embryos; cx43−/− = 13 embryos; one frame per embryo). g Qdots were microinjected into the hindbrain ventricles of WT or cx43−/− zebrafish larvae at 10 dpf and then imaged at 5 and 60 min after the microinjection. Dashed lines indicate passive migration of Qdots. Arrowheads mark the caudal end of Qdot flow. Lateral view anterior to the left. Scale bar = 1 mm. Insets represent magnifications of the dotted areas.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC AB standard conditions Long-pec ependymal cell motile cilium
IHC Days 7-13 ependymal cell motile cilium
IHC gja1bcms6/+ standard conditions Days 7-13 ependymal cell motile cilium
IHC gja1bcms6/cms6 standard conditions Long-pec ependymal cell motile cilium
IHC Days 7-13 ependymal cell motile cilium
Phenotype Details
Fish Conditions Stage Phenotype
gja1bcms6/+ standard conditions Days 7-13 ependymal cell motile cilium assembly normal occurrence, normal
Days 7-13 spinal cord has normal numbers of parts of type ependymal cell motile cilium, normal
gja1bcms6/cms6 standard conditions Long-pec ependymal cell motile cilium assembly normal occurrence, normal
Long-pec spinal cord has normal numbers of parts of type ependymal cell motile cilium, normal
Days 7-13 central canal cerebrospinal fluid circulation decreased process quality, abnormal
Days 7-13 ependymal cell motile cilium assembly decreased occurrence, abnormal
Days 7-13 spinal cord has fewer parts of type ependymal cell motile cilium, abnormal
Days 45-89 - Adult whole organism decreased size, abnormal
Days 45-89 - Adult whole organism semi-lethal (sensu genetics), abnormal
Days 45-89 - Adult whole organism sterile, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.