Figure 5

The maximum tolerated dose of TLD1433 in wild type zebrafish embryos administered through three different routes. (A) Schedule of TLD1433 treatment in wild type zebrafish. WA: TLD1433 (2.3 nM, 4.6 nM, 9.2 nM, 11.5 nM, 23 nM) were added to the water containing 10 embryos per well at 2.5, 3.5, 4.5, 5.5 dpf, for 12 h (yellow box). After these treatments, the drug was removed and replaced by egg water followed by 90 min green light irradiation (21 mW/cm², 114J.cm⁻², 520 nm), depicted as a green lightning bolt. IV or RO: 1 nL of TLD1433 (1.15 mM, 2.3 mM, 4.6 mM, 9.2 mM, 11.5 mM) were injected into the embryos at 3 dpf to 6 dpf every morning, followed by 60 min drug-to-light interval (yellow box) and 90 min green light irradiation (21 mW/cm², 114 J.cm⁻², 520 nm), depicted as a green lightning bolt. (B) WA, (C) IV, (D) RO. (B–D) Images were made of irradiated (light) and non-irradiated (dark) embryos (n = 30) at 6dpf and the percentages of mortality, malformation and fish length were calculated (shown as means ± SD from three independent experiments). Representative images of embryos under dark and light conditions are shown.